Note
Click here to download the full example code
This example demonstrates how to morph an individual subject’s
mne.SourceEstimate
to a common reference space. We achieve this using
mne.SourceMorph
. Pre-computed data will be morphed based on
a spherical representation of the cortex computed using the spherical
registration of
FreeSurfer
(https://surfer.nmr.mgh.harvard.edu/fswiki/SurfaceRegAndTemplates) [1]. This
transform will be used to morph the surface vertices of the subject towards the
reference vertices. Here we will use ‘fsaverage’ as a reference space (see
https://surfer.nmr.mgh.harvard.edu/fswiki/FsAverage).
The transformation will be applied to the surface source estimate. A plot
depicting the successful morph will be created for the spherical and inflated
surface representation of 'fsaverage'
, overlaid with the morphed surface
source estimate.
[1] | Greve D. N., Van der Haegen L., Cai Q., Stufflebeam S., Sabuncu M. R., Fischl B., Brysbaert M. A Surface-based Analysis of Language Lateralization and Cortical Asymmetry. Journal of Cognitive Neuroscience 25(9), 1477-1492, 2013. |
Note
For a tutorial about morphing see: Morphing source estimates: Moving data from one brain to another.
# Author: Tommy Clausner <tommy.clausner@gmail.com>
#
# License: BSD (3-clause)
import os
import mne
from mne.datasets import sample
print(__doc__)
Setup paths
sample_dir_raw = sample.data_path()
sample_dir = os.path.join(sample_dir_raw, 'MEG', 'sample')
subjects_dir = os.path.join(sample_dir_raw, 'subjects')
fname_stc = os.path.join(sample_dir, 'sample_audvis-meg')
Load example data
# Read stc from file
stc = mne.read_source_estimate(fname_stc, subject='sample')
In MNE surface source estimates represent the source space simply as
lists of vertices (see
The SourceEstimate data structure).
This list can either be obtained from
mne.SourceSpaces
(src) or from the stc
itself.
Since the default spacing
(resolution of surface mesh) is 5
and
subject_to
is set to ‘fsaverage’, mne.SourceMorph
will use
default ico-5 fsaverage
vertices to morph, which are the special
values [np.arange(10242)] * 2
.
Note
This is not generally true for other subjects! The set of vertices
used for fsaverage
with ico-5 spacing was designed to be
special. ico-5 spacings for other subjects (or other spacings
for fsaverage) must be calculated and will not be consecutive
integers.
If src was not defined, the morph will actually not be precomputed, because we lack the vertices from that we want to compute. Instead the morph will be set up and when applying it, the actual transformation will be computed on the fly.
Initialize SourceMorph for SourceEstimate
morph = mne.compute_source_morph(stc, subject_from='sample',
subject_to='fsaverage',
subjects_dir=subjects_dir)
Out:
surface source space inferred...
Computing morph matrix...
Left-hemisphere map read.
Right-hemisphere map read.
17 smooth iterations done.
14 smooth iterations done.
[done]
[done]
The morph will be applied to the source estimate data, by giving it as the first argument to the morph we computed above.
stc_fsaverage = morph.apply(stc)
# Define plotting parameters
surfer_kwargs = dict(
hemi='lh', subjects_dir=subjects_dir,
clim=dict(kind='value', lims=[8, 12, 15]), views='lateral',
initial_time=0.09, time_unit='s', size=(800, 800),
smoothing_steps=5)
# As spherical surface
brain = stc_fsaverage.plot(surface='sphere', **surfer_kwargs)
# Add title
brain.add_text(0.1, 0.9, 'Morphed to fsaverage (spherical)', 'title',
font_size=16)
As inflated surface
brain_inf = stc_fsaverage.plot(surface='inflated', **surfer_kwargs)
# Add title
brain_inf.add_text(0.1, 0.9, 'Morphed to fsaverage (inflated)', 'title',
font_size=16)
An instance of SourceMorph can be saved, by calling
morph.save
.
This method allows for specification of a filename under which the morph
will be save in “.h5” format. If no file extension is provided, “-morph.h5”
will be appended to the respective defined filename:
>>> morph.save('my-file-name')
Reading a saved source morph can be achieved by using
mne.read_source_morph()
:
>>> morph = mne.read_source_morph('my-file-name-morph.h5')
Once the environment is set up correctly, no information such as
subject_from
or subjects_dir
must be provided, since it can be
inferred from the data and use morph to ‘fsaverage’ by default. SourceMorph
can further be used without creating an instance and assigning it to a
variable. Instead mne.compute_source_morph()
and
mne.SourceMorph.apply()
can be
easily chained into a handy one-liner. Taking this together the shortest
possible way to morph data directly would be:
stc_fsaverage = mne.compute_source_morph(stc,
subjects_dir=subjects_dir).apply(stc)
Out:
surface source space inferred...
Computing morph matrix...
Left-hemisphere map read.
Right-hemisphere map read.
17 smooth iterations done.
14 smooth iterations done.
[done]
[done]
Total running time of the script: ( 0 minutes 34.594 seconds)
Estimated memory usage: 10 MB