Note
Click here to download the full example code
Note
This code is taken from the analysis code used in [1]. If you reuse this code please consider citing this work.
This tutorial explains how to perform a toy polysomnography analysis that answers the following question:
Important
Given two subjects from the Sleep Physionet dataset [2][3], namely Alice and Bob, how well can we predict the sleep stages of Bob from Alice’s data?
This problem is tackled as supervised multiclass classification task. The aim is to predict the sleep stage from 5 possible stages for each chunk of 30 seconds of data.
# Authors: Alexandre Gramfort <alexandre.gramfort@inria.fr>
# Stanislas Chambon <stan.chambon@gmail.com>
# Joan Massich <mailsik@gmail.com>
#
# License: BSD-3-Clause
import numpy as np
import matplotlib.pyplot as plt
import mne
from mne.datasets.sleep_physionet.age import fetch_data
from mne.time_frequency import psd_welch
from sklearn.ensemble import RandomForestClassifier
from sklearn.metrics import accuracy_score
from sklearn.metrics import confusion_matrix
from sklearn.metrics import classification_report
from sklearn.pipeline import make_pipeline
from sklearn.preprocessing import FunctionTransformer
Here we download the data from two subjects and the end goal is to obtain epochs and its associated ground truth.
MNE-Python provides us with
mne.datasets.sleep_physionet.age.fetch_data()
to conveniently download
data from the Sleep Physionet dataset
[2][3].
Given a list of subjects and records, the fetcher downloads the data and
provides us for each subject, a pair of files:
-PSG.edf
containing the polysomnography. The raw data from the
EEG helmet,
-Hypnogram.edf
containing the annotations recorded by an
expert.
Combining these two in a mne.io.Raw
object then we can extract
events based on the descriptions of the annotations to obtain the
epochs.
ALICE, BOB = 0, 1
[alice_files, bob_files] = fetch_data(subjects=[ALICE, BOB], recording=[1])
raw_train = mne.io.read_raw_edf(alice_files[0], stim_channel='Event marker',
misc=['Temp rectal'])
annot_train = mne.read_annotations(alice_files[1])
raw_train.set_annotations(annot_train, emit_warning=False)
# plot some data
# scalings were chosen manually to allow for simultaneous visualization of
# different channel types in this specific dataset
raw_train.plot(start=60, duration=60,
scalings=dict(eeg=1e-4, resp=1e3, eog=1e-4, emg=1e-7,
misc=1e-1))
Using default location ~/mne_data for PHYSIONET_SLEEP...
Extracting EDF parameters from /home/circleci/mne_data/physionet-sleep-data/SC4001E0-PSG.edf...
EDF file detected
Setting channel info structure...
Creating raw.info structure...
The Sleep Physionet dataset is annotated using 8 labels: Wake (W), Stage 1, Stage 2, Stage 3, Stage 4 corresponding to the range from light sleep to deep sleep, REM sleep (R) where REM is the abbreviation for Rapid Eye Movement sleep, movement (M), and Stage (?) for any none scored segment.
We will work only with 5 stages: Wake (W), Stage 1, Stage 2, Stage 3/4, and
REM sleep (R). To do so, we use the event_id
parameter in
mne.events_from_annotations()
to select which events are we
interested in and we associate an event identifier to each of them.
Moreover, the recordings contain long awake (W) regions before and after each night. To limit the impact of class imbalance, we trim each recording by only keeping 30 minutes of wake time before the first occurrence and 30 minutes after the last occurrence of sleep stages.
annotation_desc_2_event_id = {'Sleep stage W': 1,
'Sleep stage 1': 2,
'Sleep stage 2': 3,
'Sleep stage 3': 4,
'Sleep stage 4': 4,
'Sleep stage R': 5}
# keep last 30-min wake events before sleep and first 30-min wake events after
# sleep and redefine annotations on raw data
annot_train.crop(annot_train[1]['onset'] - 30 * 60,
annot_train[-2]['onset'] + 30 * 60)
raw_train.set_annotations(annot_train, emit_warning=False)
events_train, _ = mne.events_from_annotations(
raw_train, event_id=annotation_desc_2_event_id, chunk_duration=30.)
# create a new event_id that unifies stages 3 and 4
event_id = {'Sleep stage W': 1,
'Sleep stage 1': 2,
'Sleep stage 2': 3,
'Sleep stage 3/4': 4,
'Sleep stage R': 5}
# plot events
fig = mne.viz.plot_events(events_train, event_id=event_id,
sfreq=raw_train.info['sfreq'],
first_samp=events_train[0, 0])
# keep the color-code for further plotting
stage_colors = plt.rcParams['axes.prop_cycle'].by_key()['color']
Used Annotations descriptions: ['Sleep stage 1', 'Sleep stage 2', 'Sleep stage 3', 'Sleep stage 4', 'Sleep stage R', 'Sleep stage W']
tmax = 30. - 1. / raw_train.info['sfreq'] # tmax in included
epochs_train = mne.Epochs(raw=raw_train, events=events_train,
event_id=event_id, tmin=0., tmax=tmax, baseline=None)
print(epochs_train)
Not setting metadata
841 matching events found
No baseline correction applied
0 projection items activated
<Epochs | 841 events (good & bad), 0 - 29.99 sec, baseline off, ~12 kB, data not loaded,
'Sleep stage W': 188
'Sleep stage 1': 58
'Sleep stage 2': 250
'Sleep stage 3/4': 220
'Sleep stage R': 125>
raw_test = mne.io.read_raw_edf(bob_files[0], stim_channel='Event marker',
misc=['Temp rectal'])
annot_test = mne.read_annotations(bob_files[1])
annot_test.crop(annot_test[1]['onset'] - 30 * 60,
annot_test[-2]['onset'] + 30 * 60)
raw_test.set_annotations(annot_test, emit_warning=False)
events_test, _ = mne.events_from_annotations(
raw_test, event_id=annotation_desc_2_event_id, chunk_duration=30.)
epochs_test = mne.Epochs(raw=raw_test, events=events_test, event_id=event_id,
tmin=0., tmax=tmax, baseline=None)
print(epochs_test)
Extracting EDF parameters from /home/circleci/mne_data/physionet-sleep-data/SC4011E0-PSG.edf...
EDF file detected
Setting channel info structure...
Creating raw.info structure...
Used Annotations descriptions: ['Sleep stage 1', 'Sleep stage 2', 'Sleep stage 3', 'Sleep stage 4', 'Sleep stage R', 'Sleep stage W']
Not setting metadata
1103 matching events found
No baseline correction applied
0 projection items activated
<Epochs | 1103 events (good & bad), 0 - 29.99 sec, baseline off, ~12 kB, data not loaded,
'Sleep stage W': 157
'Sleep stage 1': 109
'Sleep stage 2': 562
'Sleep stage 3/4': 105
'Sleep stage R': 170>
Observing the power spectral density (PSD) plot of the epochs grouped by sleeping stage we can see that different sleep stages have different signatures. These signatures remain similar between Alice and Bob’s data.
The rest of this section we will create EEG features based on relative power in specific frequency bands to capture this difference between the sleep stages in our data.
# visualize Alice vs. Bob PSD by sleep stage.
fig, (ax1, ax2) = plt.subplots(ncols=2)
# iterate over the subjects
stages = sorted(event_id.keys())
for ax, title, epochs in zip([ax1, ax2],
['Alice', 'Bob'],
[epochs_train, epochs_test]):
for stage, color in zip(stages, stage_colors):
epochs[stage].plot_psd(area_mode=None, color=color, ax=ax,
fmin=0.1, fmax=20., show=False,
average=True, spatial_colors=False)
ax.set(title=title, xlabel='Frequency (Hz)')
ax2.set(ylabel='µV^2/Hz (dB)')
ax2.legend(ax2.lines[2::3], stages)
plt.show()
Loading data for 58 events and 3000 original time points ...
0 bad epochs dropped
Using multitaper spectrum estimation with 7 DPSS windows
Loading data for 250 events and 3000 original time points ...
0 bad epochs dropped
Using multitaper spectrum estimation with 7 DPSS windows
Loading data for 220 events and 3000 original time points ...
0 bad epochs dropped
Using multitaper spectrum estimation with 7 DPSS windows
Loading data for 125 events and 3000 original time points ...
0 bad epochs dropped
Using multitaper spectrum estimation with 7 DPSS windows
Loading data for 188 events and 3000 original time points ...
0 bad epochs dropped
Using multitaper spectrum estimation with 7 DPSS windows
Loading data for 109 events and 3000 original time points ...
0 bad epochs dropped
Using multitaper spectrum estimation with 7 DPSS windows
Loading data for 562 events and 3000 original time points ...
0 bad epochs dropped
Using multitaper spectrum estimation with 7 DPSS windows
Loading data for 105 events and 3000 original time points ...
0 bad epochs dropped
Using multitaper spectrum estimation with 7 DPSS windows
Loading data for 170 events and 3000 original time points ...
0 bad epochs dropped
Using multitaper spectrum estimation with 7 DPSS windows
Loading data for 157 events and 3000 original time points ...
0 bad epochs dropped
Using multitaper spectrum estimation with 7 DPSS windows
We will now create a function to extract EEG features based on relative power in specific frequency bands to be able to predict sleep stages from EEG signals.
def eeg_power_band(epochs):
"""EEG relative power band feature extraction.
This function takes an ``mne.Epochs`` object and creates EEG features based
on relative power in specific frequency bands that are compatible with
scikit-learn.
Parameters
----------
epochs : Epochs
The data.
Returns
-------
X : numpy array of shape [n_samples, 5]
Transformed data.
"""
# specific frequency bands
FREQ_BANDS = {"delta": [0.5, 4.5],
"theta": [4.5, 8.5],
"alpha": [8.5, 11.5],
"sigma": [11.5, 15.5],
"beta": [15.5, 30]}
psds, freqs = psd_welch(epochs, picks='eeg', fmin=0.5, fmax=30.)
# Normalize the PSDs
psds /= np.sum(psds, axis=-1, keepdims=True)
X = []
for fmin, fmax in FREQ_BANDS.values():
psds_band = psds[:, :, (freqs >= fmin) & (freqs < fmax)].mean(axis=-1)
X.append(psds_band.reshape(len(psds), -1))
return np.concatenate(X, axis=1)
To answer the question of how well can we predict the sleep stages of Bob from Alice’s data and avoid as much boilerplate code as possible, we will take advantage of two key features of sckit-learn: Pipeline , and FunctionTransformer.
Scikit-learn pipeline composes an estimator as a sequence of transforms
and a final estimator, while the FunctionTransformer converts a python
function in an estimator compatible object. In this manner we can create
scikit-learn estimator that takes mne.Epochs
thanks to
eeg_power_band
function we just created.
pipe = make_pipeline(FunctionTransformer(eeg_power_band, validate=False),
RandomForestClassifier(n_estimators=100, random_state=42))
# Train
y_train = epochs_train.events[:, 2]
pipe.fit(epochs_train, y_train)
# Test
y_pred = pipe.predict(epochs_test)
# Assess the results
y_test = epochs_test.events[:, 2]
acc = accuracy_score(y_test, y_pred)
print("Accuracy score: {}".format(acc))
Loading data for 841 events and 3000 original time points ...
0 bad epochs dropped
Effective window size : 2.560 (s)
[Parallel(n_jobs=1)]: Using backend SequentialBackend with 1 concurrent workers.
[Parallel(n_jobs=1)]: Done 1 out of 1 | elapsed: 1.5s remaining: 0.0s
[Parallel(n_jobs=1)]: Done 1 out of 1 | elapsed: 1.5s finished
Loading data for 1103 events and 3000 original time points ...
0 bad epochs dropped
Effective window size : 2.560 (s)
[Parallel(n_jobs=1)]: Using backend SequentialBackend with 1 concurrent workers.
[Parallel(n_jobs=1)]: Done 1 out of 1 | elapsed: 1.8s remaining: 0.0s
[Parallel(n_jobs=1)]: Done 1 out of 1 | elapsed: 1.8s finished
Accuracy score: 0.641885766092475
In short, yes. We can predict Bob’s sleeping stages based on Alice’s data.
We can check the confusion matrix or the classification report.
print(confusion_matrix(y_test, y_pred))
[[156 0 1 0 0]
[ 80 4 7 2 16]
[ 85 17 369 33 58]
[ 0 0 5 100 0]
[ 54 3 34 0 79]]
print(classification_report(y_test, y_pred, target_names=event_id.keys()))
precision recall f1-score support
Sleep stage W 0.42 0.99 0.59 157
Sleep stage 1 0.17 0.04 0.06 109
Sleep stage 2 0.89 0.66 0.75 562
Sleep stage 3/4 0.74 0.95 0.83 105
Sleep stage R 0.52 0.46 0.49 170
accuracy 0.64 1103
macro avg 0.55 0.62 0.54 1103
weighted avg 0.68 0.64 0.63 1103
Fetch 50 subjects from the Physionet database and run a 5-fold cross-validation leaving each time 10 subjects out in the test set.
Total running time of the script: ( 0 minutes 43.449 seconds)
Estimated memory usage: 266 MB