Visualizing Evoked data

This tutorial shows the different visualization methods for Evoked objects.

As usual we’ll start by importing the modules we need:

import numpy as np

import mne

Instead of creating the Evoked object from an Epochs object, we’ll load an existing Evoked object from disk. Remember, the .fif format can store multiple Evoked objects, so we’ll end up with a list of Evoked objects after loading. Recall also from the Loading and saving Evoked data section of the introductory Evoked tutorial that the sample Evoked objects have not been baseline-corrected and have unapplied projectors, so we’ll take care of that when loading:

root = mne.datasets.sample.data_path() / 'MEG' / 'sample'
evk_file = root / 'sample_audvis-ave.fif'
evokeds_list = mne.read_evokeds(evk_file, baseline=(None, 0), proj=True,
                                verbose=False)

# Show condition names and baseline intervals
for e in evokeds_list:
    print(f'Condition: {e.comment}, baseline: {e.baseline}')

Condition: Left Auditory, baseline: (-0.19979521315838786, 0.0)
Condition: Right Auditory, baseline: (-0.19979521315838786, 0.0)
Condition: Left visual, baseline: (-0.19979521315838786, 0.0)
Condition: Right visual, baseline: (-0.19979521315838786, 0.0)

To make our life easier, let’s convert that list of Evoked objects into a dictionary. We’ll use /-separated dictionary keys to encode the conditions (like is often done when epoching) because some of the plotting methods can take advantage of that style of coding.

conds = ('aud/left', 'aud/right', 'vis/left', 'vis/right')
evks = dict(zip(conds, evokeds_list))
#      ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^ this is equivalent to:
# {'aud/left': evokeds_list[0], 'aud/right': evokeds_list[1],
#  'vis/left': evokeds_list[2], 'vis/right': evokeds_list[3]}

Plotting signal traces

Butterfly plots

Plots of superimposed sensor timeseries are called “butterfly plots” because the positive- and negative-going traces can resemble butterfly wings.

The most basic plot of Evoked objects is a butterfly plot of each channel type, generated by the evoked.plot() method. By default, channels marked as “bad” are suppressed, but you can control this by passing an empty list to the exclude parameter (default is exclude='bads'):

evks['aud/left'].plot(exclude=[])

Notice the completely flat EEG channel and the noisy gradiometer channel plotted in red color. Like many MNE-Python plotting functions, evoked.plot() has a picks parameter that can select channels to plot by name, index, or type. In the next plot, we’ll show only magnetometer channels and also color-code the channel traces by their location by passing spatial_colors=True. Finally, we’ll superimpose a trace of the root mean square (RMS) of the signal across channels by passing gfp=True. This parameter is called gfp for historical reasons and behaves correctly for all supported channel types: for MEG data, it will plot the RMS; while for EEG, it would plot the global field power (an average-referenced RMS), hence its name:

evks['aud/left'].plot(picks='mag', spatial_colors=True, gfp=True)

Interesting time periods can be highlighted via the highlight parameter.

time_ranges_of_interest = [
    (0.05, 0.14),
    (0.22, 0.27)
]
evks['aud/left'].plot(
    picks='mag', spatial_colors=True, gfp=True,
    highlight=time_ranges_of_interest
)

Plotting scalp topographies

In an interactive session, the butterfly plots seen above can be click-dragged to select a time region, which will pop up a map of the average field distribution over the scalp for the selected time span. You can also generate scalp topographies at specific times or time spans using the plot_topomap() method:

times = np.linspace(0.05, 0.13, 5)
evks['aud/left'].plot_topomap(ch_type='mag', times=times, colorbar=True)

fig = evks['aud/left'].plot_topomap(ch_type='mag', times=times, average=0.1)

It is also possible to pass different time durations to average over for each time point. Passing a value of None will disable averaging for that time point:

averaging_durations = [0.01, 0.02, 0.03, None, None]
fig = evks['aud/left'].plot_topomap(
    ch_type='mag', times=times, average=averaging_durations
)

Additional examples of plotting scalp topographies can be found in Plotting topographic maps of evoked data.

Arrow maps

Scalp topographies at a given time point can be augmented with arrows to show the estimated magnitude and direction of the magnetic field, using the function mne.viz.plot_arrowmap():

mags = evks['aud/left'].copy().pick_types(meg='mag')
mne.viz.plot_arrowmap(mags.data[:, 175], mags.info, extrapolate='local')

Removing projector <Projection | Average EEG reference, active : True, n_channels : 60>

Joint plots

Joint plots combine butterfly plots with scalp topographies, and provide an excellent first-look at evoked data; by default, topographies will be automatically placed based on peak finding. Here we plot the right-visual-field condition; if no picks are specified we get a separate figure for each channel type:

evks['vis/right'].plot_joint()

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Projections have already been applied. Setting proj attribute to True.
Removing projector <Projection | PCA-v1, active : True, n_channels : 102>
Removing projector <Projection | PCA-v2, active : True, n_channels : 102>
Removing projector <Projection | PCA-v3, active : True, n_channels : 102>
Removing projector <Projection | Average EEG reference, active : True, n_channels : 60>
Removing projector <Projection | Average EEG reference, active : True, n_channels : 60>
Removing projector <Projection | PCA-v1, active : True, n_channels : 102>
Removing projector <Projection | PCA-v2, active : True, n_channels : 102>
Removing projector <Projection | PCA-v3, active : True, n_channels : 102>

Like plot_topomap(), you can specify the times at which you want the scalp topographies calculated, and you can customize the plot in various other ways as well. See mne.Evoked.plot_joint() for details.

Comparing Evoked objects

To compare Evoked objects from different experimental conditions, the function mne.viz.plot_compare_evokeds() can take a list or dict of Evoked objects and plot them all on the same axes. Like most MNE-Python visualization functions, it has a picks parameter for selecting channels, but by default will generate one figure for each channel type, and combine information across channels of the same type by calculating the global field power. Information may be combined across channels in other ways too; support for combining via mean, median, or standard deviation are built-in, and custom callable functions may also be used, as shown here:

def custom_func(x):
    return x.max(axis=1)


for combine in ('mean', 'median', 'gfp', custom_func):
    mne.viz.plot_compare_evokeds(evks, picks='eeg', combine=combine)

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• 

combining channels using "mean"
combining channels using "mean"
combining channels using "mean"
combining channels using "mean"
combining channels using "median"
combining channels using "median"
combining channels using "median"
combining channels using "median"
combining channels using "gfp"
combining channels using "gfp"
combining channels using "gfp"
combining channels using "gfp"
combining channels using "<function custom_func at 0x7fbf57182ef0>"
combining channels using "<function custom_func at 0x7fbf57182ef0>"
combining channels using "<function custom_func at 0x7fbf57182ef0>"
combining channels using "<function custom_func at 0x7fbf57182ef0>"

One nice feature of plot_compare_evokeds() is that when passing evokeds in a dictionary, it allows specifying plot styles based on /-separated substrings of the dictionary keys (similar to epoch selection; see Subselecting epochs). Here, we specify colors for “aud” and “vis” conditions, and linestyles for “left” and “right” conditions, and the traces and legend are styled accordingly. Here we also show the time_unit='ms' parameter in action.

mne.viz.plot_compare_evokeds(evks, picks='MEG 1811', colors=dict(aud=0, vis=1),
                             linestyles=dict(left='solid', right='dashed'),
                             time_unit='ms')

The legends generated by plot_compare_evokeds() above used the dictionary keys provided by the evks variable. If instead you pass a list or tuple of Evoked objects, the legend keys will be generated automatically from the comment attribute of the Evoked objects (or, as sequential integers if the comment attribute is empty or ambiguous). To illustrate this, we’ll make a list of five Evoked objects: two with identical comments, two with empty comments (either an empty string or None), and one with a unique non-empty comment:

temp_list = list()
for idx, _comment in enumerate(('foo', 'foo', '', None, 'bar'), start=1):
    _evk = evokeds_list[0].copy()
    _evk.comment = _comment
    _evk.data *= idx  # so we can tell the traces apart
    temp_list.append(_evk)

mne.viz.plot_compare_evokeds(temp_list, picks='mag')

combining channels using "gfp"
combining channels using "gfp"
combining channels using "gfp"
combining channels using "gfp"
combining channels using "gfp"

Image plots

Like Epochs, Evoked objects also have a plot_image() method, but unlike epochs.plot_image(), evoked.plot_image() shows one channel per row instead of one epoch per row. Again, a picks parameter is available, as well as several other customization options; see plot_image() for details.

evks['vis/right'].plot_image(picks='meg')

Topographical subplots

For sensor-level analyses, it can be useful to plot the response at each sensor in a topographical layout. The plot_compare_evokeds() function can do this if you pass axes='topo', but it can be quite slow if the number of sensors is too large, so here we’ll plot only the EEG channels:

mne.viz.plot_compare_evokeds(evks, picks='eeg', colors=dict(aud=0, vis=1),
                             linestyles=dict(left='solid', right='dashed'),
                             axes='topo', styles=dict(aud=dict(linewidth=1),
                                                      vis=dict(linewidth=1)))

For a larger number of sensors, the method evoked.plot_topo() and the function mne.viz.plot_evoked_topo() can both be used. The plot_topo() method will plot only a single condition, while the plot_evoked_topo() function can plot one or more conditions on the same axes, if passed a list of Evoked objects. The legend entries will be automatically drawn from the Evoked objects’ comment attribute:

mne.viz.plot_evoked_topo(evokeds_list)

By default, plot_evoked_topo() will plot all MEG sensors (if present), so to get EEG sensors you would need to modify the evoked objects first (e.g., using mne.pick_types).

Note

In interactive sessions, both approaches to topographical plotting allow you to click one of the sensor subplots to open a larger version of the evoked plot at that sensor.

3D Field Maps

The scalp topographies above were all projected into two-dimensional overhead views of the field, but it is also possible to plot field maps in 3D. This requires a trans file to transform locations between the coordinate systems of the MEG device and the head surface (based on the MRI). You can compute 3D field maps without a trans file, but it will only work for calculating the field on the MEG helmet from the MEG sensors.

subjects_dir = root.parent.parent / 'subjects'
trans_file = root / 'sample_audvis_raw-trans.fif'

By default, MEG sensors will be used to estimate the field on the helmet surface, while EEG sensors will be used to estimate the field on the scalp. Once the maps are computed, you can plot them with evoked.plot_field():

maps = mne.make_field_map(evks['aud/left'], trans=str(trans_file),
                          subject='sample', subjects_dir=subjects_dir)
evks['aud/left'].plot_field(maps, time=0.1)

Using surface from /home/circleci/mne_data/MNE-sample-data/subjects/sample/bem/sample-5120-5120-5120-bem.fif.
Getting helmet for system 306m
Prepare EEG mapping...
Computing dot products for 59 electrodes...
[Parallel(n_jobs=1)]: Using backend SequentialBackend with 1 concurrent workers.
[Parallel(n_jobs=1)]: Done   1 out of   1 | elapsed:    0.0s remaining:    0.0s
[Parallel(n_jobs=1)]: Done   1 out of   1 | elapsed:    0.0s finished
Computing dot products for 2562 surface locations...
[Parallel(n_jobs=1)]: Using backend SequentialBackend with 1 concurrent workers.
[Parallel(n_jobs=1)]: Done   1 out of   1 | elapsed:    0.3s remaining:    0.0s
[Parallel(n_jobs=1)]: Done   1 out of   1 | elapsed:    0.3s finished
Field mapping data ready
    Preparing the mapping matrix...
    Truncating at 21/59 components to omit less than 0.001 (0.00097)
    The map has an average electrode reference (2562 channels)
Prepare MEG mapping...
Computing dot products for 305 coils...
[Parallel(n_jobs=1)]: Using backend SequentialBackend with 1 concurrent workers.
[Parallel(n_jobs=1)]: Done   1 out of   1 | elapsed:    1.1s remaining:    0.0s
[Parallel(n_jobs=1)]: Done   1 out of   1 | elapsed:    1.1s finished
Computing dot products for 304 surface locations...
[Parallel(n_jobs=1)]: Using backend SequentialBackend with 1 concurrent workers.
[Parallel(n_jobs=1)]: Done   1 out of   1 | elapsed:    0.8s remaining:    0.0s
[Parallel(n_jobs=1)]: Done   1 out of   1 | elapsed:    0.8s finished
Field mapping data ready
    Preparing the mapping matrix...
    Truncating at 210/305 components to omit less than 0.0001 (9.9e-05)

You can also use MEG sensors to estimate the scalp field by passing meg_surf='head'. By selecting each sensor type in turn, you can compare the scalp field estimates from each.

for ch_type in ('mag', 'grad', 'eeg'):
    evk = evks['aud/right'].copy().pick(ch_type)
    _map = mne.make_field_map(evk, trans=str(trans_file), subject='sample',
                              subjects_dir=subjects_dir, meg_surf='head')
    fig = evk.plot_field(_map, time=0.1)
    mne.viz.set_3d_title(fig, ch_type, size=20)

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Removing projector <Projection | Average EEG reference, active : True, n_channels : 60>
Using surface from /home/circleci/mne_data/MNE-sample-data/subjects/sample/bem/sample-5120-5120-5120-bem.fif.
Prepare MEG mapping...
Computing dot products for 102 coils...
[Parallel(n_jobs=1)]: Using backend SequentialBackend with 1 concurrent workers.
[Parallel(n_jobs=1)]: Done   1 out of   1 | elapsed:    0.2s remaining:    0.0s
[Parallel(n_jobs=1)]: Done   1 out of   1 | elapsed:    0.2s finished
Computing dot products for 2562 surface locations...
[Parallel(n_jobs=1)]: Using backend SequentialBackend with 1 concurrent workers.
[Parallel(n_jobs=1)]: Done   1 out of   1 | elapsed:    3.1s remaining:    0.0s
[Parallel(n_jobs=1)]: Done   1 out of   1 | elapsed:    3.1s finished
Field mapping data ready
    Preparing the mapping matrix...
    Truncating at 57/102 components to omit less than 0.0001 (9.9e-05)
Removing projector <Projection | PCA-v1, active : True, n_channels : 102>
Removing projector <Projection | PCA-v2, active : True, n_channels : 102>
Removing projector <Projection | PCA-v3, active : True, n_channels : 102>
Removing projector <Projection | Average EEG reference, active : True, n_channels : 60>
Using surface from /home/circleci/mne_data/MNE-sample-data/subjects/sample/bem/sample-5120-5120-5120-bem.fif.
Prepare MEG mapping...
Computing dot products for 203 coils...
[Parallel(n_jobs=1)]: Using backend SequentialBackend with 1 concurrent workers.
[Parallel(n_jobs=1)]: Done   1 out of   1 | elapsed:    0.7s remaining:    0.0s
[Parallel(n_jobs=1)]: Done   1 out of   1 | elapsed:    0.7s finished
Computing dot products for 2562 surface locations...
[Parallel(n_jobs=1)]: Using backend SequentialBackend with 1 concurrent workers.
[Parallel(n_jobs=1)]: Done   1 out of   1 | elapsed:    4.4s remaining:    0.0s
[Parallel(n_jobs=1)]: Done   1 out of   1 | elapsed:    4.4s finished
Field mapping data ready
    Preparing the mapping matrix...
    Truncating at 166/203 components to omit less than 0.0001 (9.7e-05)
Removing projector <Projection | PCA-v1, active : True, n_channels : 102>
Removing projector <Projection | PCA-v2, active : True, n_channels : 102>
Removing projector <Projection | PCA-v3, active : True, n_channels : 102>
Using surface from /home/circleci/mne_data/MNE-sample-data/subjects/sample/bem/sample-5120-5120-5120-bem.fif.
Prepare EEG mapping...
Computing dot products for 59 electrodes...
[Parallel(n_jobs=1)]: Using backend SequentialBackend with 1 concurrent workers.
[Parallel(n_jobs=1)]: Done   1 out of   1 | elapsed:    0.0s remaining:    0.0s
[Parallel(n_jobs=1)]: Done   1 out of   1 | elapsed:    0.0s finished
Computing dot products for 2562 surface locations...
[Parallel(n_jobs=1)]: Using backend SequentialBackend with 1 concurrent workers.
[Parallel(n_jobs=1)]: Done   1 out of   1 | elapsed:    0.2s remaining:    0.0s
[Parallel(n_jobs=1)]: Done   1 out of   1 | elapsed:    0.2s finished
Field mapping data ready
    Preparing the mapping matrix...
    Truncating at 21/59 components to omit less than 0.001 (0.00097)
    The map has an average electrode reference (2562 channels)

Estimated memory usage: 30 MB