Convert NIRS data to BIDS format#

In this example, we use MNE-BIDS to create a BIDS-compatible directory of NIRS data. Specifically, we will follow these steps:

  1. Download some NIRS data

  2. Load the data, extract information, and save it in a new BIDS directory.

  3. Check the result and compare it with the standard.

  4. Cite mne-bids.

# Authors: The MNE-BIDS developers
# SPDX-License-Identifier: BSD-3-Clause

We are importing everything we need for this example:

import os.path as op
import pathlib
import shutil

import mne
from mne_nirs import datasets  # For convenient downloading of example data

from mne_bids import BIDSPath, print_dir_tree, write_raw_bids
from mne_bids.stats import count_events

Download the data#

First, we need some data to work with. We will use the Finger Tapping Dataset available on GitHub. We will use the MNE-NIRS package which includes convenient functions to download openly available datasets.

data_dir = pathlib.Path(datasets.fnirs_motor_group.data_path())

# Let's see whether the data has been downloaded using a quick visualization
# of the directory tree.
print_dir_tree(data_dir)
Using default location ~/mne_data for fnirs_motor_group...

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Download complete in 03s (41.7 MB)
|fNIRS-motor-group/
|--- .gitignore
|--- CITATION.cff
|--- README.md
|--- dataset_description.json
|--- participants.json
|--- participants.tsv
|--- sub-01/
|------ sub-01_scans.tsv
|------ nirs/
|--------- sub-01_coordsystem.json
|--------- sub-01_optodes.tsv
|--------- sub-01_task-tapping_channels.tsv
|--------- sub-01_task-tapping_events.tsv
|--------- sub-01_task-tapping_nirs.json
|--------- sub-01_task-tapping_nirs.snirf
|--- sub-02/
|------ sub-02_scans.tsv
|------ nirs/
|--------- sub-02_coordsystem.json
|--------- sub-02_optodes.tsv
|--------- sub-02_task-tapping_channels.tsv
|--------- sub-02_task-tapping_events.tsv
|--------- sub-02_task-tapping_nirs.json
|--------- sub-02_task-tapping_nirs.snirf
|--- sub-03/
|------ sub-03_scans.tsv
|------ nirs/
|--------- sub-03_coordsystem.json
|--------- sub-03_optodes.tsv
|--------- sub-03_task-tapping_channels.tsv
|--------- sub-03_task-tapping_events.tsv
|--------- sub-03_task-tapping_nirs.json
|--------- sub-03_task-tapping_nirs.snirf
|--- sub-04/
|------ sub-04_scans.tsv
|------ nirs/
|--------- sub-04_coordsystem.json
|--------- sub-04_optodes.tsv
|--------- sub-04_task-tapping_channels.tsv
|--------- sub-04_task-tapping_events.tsv
|--------- sub-04_task-tapping_nirs.json
|--------- sub-04_task-tapping_nirs.snirf
|--- sub-05/
|------ sub-05_scans.tsv
|------ nirs/
|--------- sub-05_coordsystem.json
|--------- sub-05_optodes.tsv
|--------- sub-05_task-tapping_channels.tsv
|--------- sub-05_task-tapping_events.tsv
|--------- sub-05_task-tapping_nirs.json
|--------- sub-05_task-tapping_nirs.snirf

The data are already in BIDS format. However, we will just use one of the SNIRF files and demonstrate how this could be used to generate a new BIDS compliant dataset from this single file.

# Specify file to use as input to BIDS generation process
file_path = data_dir / "sub-01" / "nirs" / "sub-01_task-tapping_nirs.snirf"

Convert to BIDS#

Let’s start with loading the data and updating the annotations. We are reading the data using MNE-Python’s io module and the mne.io.read_raw_snirf() function. Note that we must use the preload=False parameter, which is the default in MNE-Python. It prevents the data from being loaded and modified when converting to BIDS.

# Load the data
raw = mne.io.read_raw_snirf(file_path, preload=False)
raw.info["line_freq"] = 50  # specify power line frequency as required by BIDS

# Sanity check, show the optode positions
raw.plot_sensors()
convert nirs to bids
Loading /home/runner/mne_data/fNIRS-motor-group/sub-01/nirs/sub-01_task-tapping_nirs.snirf

<Figure size 640x640 with 1 Axes>

I also like to rename the annotations to something meaningful and set the duration of each stimulus

trigger_info = {"1.0": "Control", "2.0": "Tapping/Left", "3.0": "Tapping/Right"}
raw.annotations.rename(trigger_info)
raw.annotations.set_durations(5.0)
<Annotations | 92 segments: 15.0 (2), Control (30), Tapping/Left (30), ...>

With these steps, we have everything to start a new BIDS directory using our data.

To do that, we can use write_raw_bids()

Generally, write_raw_bids() tries to extract as much meta data as possible from the raw data and then formats it in a BIDS compatible way. write_raw_bids() takes a bunch of inputs, most of which are however optional. The required inputs are:

  • raw

  • bids_basename

  • bids_root

… as you can see in the docstring:

print(write_raw_bids.__doc__)

# zero padding to account for >100 subjects in this dataset
subject_id = "01"

# define a task name and a directory where to save the data to
task = "Tapping"
bids_root = data_dir.with_name(data_dir.name + "-bids")
print(bids_root)
Save raw data to a BIDS-compliant folder structure.

    .. warning:: * The original file is simply copied over if the original
                   file format is BIDS-supported for that datatype. Otherwise,
                   this function will convert to a BIDS-supported file format
                   while warning the user. For EEG and iEEG data, conversion
                   will be to BrainVision format; for MEG, conversion will be
                   to FIFF.

                 * ``mne-bids`` will infer the manufacturer information
                   from the file extension. If your file format is non-standard
                   for the manufacturer, please update the manufacturer field
                   in the sidecars manually.

    Parameters
    ----------
    raw : mne.io.Raw
        The raw data. It must be an instance of `mne.io.Raw` that is not
        already loaded from disk unless ``allow_preload`` is explicitly set
        to ``True``. See warning for the ``allow_preload`` parameter.
    bids_path : BIDSPath
        The file to write. The :class:`mne_bids.BIDSPath` instance passed here
        **must** have the ``subject``, ``task``, and ``root`` attributes set.
        If the ``datatype`` attribute is not set, it will be inferred from the
        recording data type found in ``raw``. In case of multiple data types,
        the ``.datatype`` attribute must be set.
        Example::

            bids_path = BIDSPath(subject='01', session='01', task='testing',
                                 acquisition='01', run='01', datatype='meg',
                                 root='/data/BIDS')

        This will write the following files in the correct subfolder ``root``::

            sub-01_ses-01_task-testing_acq-01_run-01_meg.fif
            sub-01_ses-01_task-testing_acq-01_run-01_meg.json
            sub-01_ses-01_task-testing_acq-01_run-01_channels.tsv
            sub-01_ses-01_acq-01_coordsystem.json

        and the following one if ``events`` is not ``None``::

            sub-01_ses-01_task-testing_acq-01_run-01_events.tsv

        and add a line to the following files::

            participants.tsv
            scans.tsv

        Note that the extension is automatically inferred from the raw
        object.
    events : path-like | np.ndarray | None
        Use this parameter to specify events to write to the ``*_events.tsv``
        sidecar file, additionally to the object's :class:`~mne.Annotations`
        (which are always written).
        If ``path-like``, specifies the location of an MNE events file.
        If an array, the MNE events array (shape: ``(n_events, 3)``).
        If a path or an array and ``raw.annotations`` exist, the union of
        ``events`` and ``raw.annotations`` will be written.
        Mappings from event names to event codes (listed in the third
        column of the MNE events array) must be specified via the ``event_id``
        parameter; otherwise, an exception is raised. If
        :class:`~mne.Annotations` are present, their descriptions must be
        included in ``event_id`` as well.
        If ``None``, events will only be inferred from the raw object's
        :class:`~mne.Annotations`.

        .. note::
           If specified, writes the union of ``events`` and
           ``raw.annotations``. If you wish to **only** write
           ``raw.annotations``, pass ``events=None``. If you want to
           **exclude** the events in ``raw.annotations`` from being written,
           call ``raw.set_annotations(None)`` before invoking this function.

        .. note::
           Either, descriptions of all event codes must be specified via the
           ``event_id`` parameter or each event must be accompanied by a
           row in ``event_metadata``.

    event_id : dict | None
        Descriptions or names describing the event codes, if you passed
        ``events``. The descriptions will be written to the ``trial_type``
        column in ``*_events.tsv``. The dictionary keys correspond to the event
        description,s and the values to the event codes. You must specify a
        description for all event codes appearing in ``events``. If your data
        contains :class:`~mne.Annotations`, you can use this parameter to
        assign event codes to each unique annotation description (mapping from
        description to event code).
    event_metadata : pandas.DataFrame | None
        Metadata for each event in ``events``. Each row corresponds to an event.
    extra_columns_descriptions : dict | None
        A dictionary that maps column names of the ``event_metadata`` to descriptions.
        Each column of ``event_metadata`` must have a corresponding entry in this.
    anonymize : dict | None
        If `None` (default), no anonymization is performed.
        If a dictionary, data will be anonymized depending on the dictionary
        keys: ``daysback`` is a required key, ``keep_his`` is optional.

        ``daysback`` : int
            Number of days by which to move back the recording date in time.
            In studies with multiple subjects the relative recording date
            differences between subjects can be kept by using the same number
            of ``daysback`` for all subject anonymizations. ``daysback`` should
            be great enough to shift the date prior to 1925 to conform with
            BIDS anonymization rules.

        ``keep_his`` : bool
            If ``False`` (default), all subject information next to the
            recording date will be overwritten as well. If ``True``, keep
            subject information apart from the recording date.

        ``keep_source`` : bool
            Whether to store the name of the ``raw`` input file in the
            ``source`` column of ``scans.tsv``. By default, this information
            is not stored.

    format : 'auto' | 'BrainVision' | 'EDF' | 'FIF' | 'EEGLAB'
        Controls the file format of the data after BIDS conversion. If
        ``'auto'``, MNE-BIDS will attempt to convert the input data to BIDS
        without a change of the original file format. A conversion to a
        different file format will then only take place if the original file
        format lacks some necessary features.
        Conversion may be forced to BrainVision, EDF, or EEGLAB for (i)EEG,
        and to FIF for MEG data.
    symlink : bool
        Instead of copying the source files, only create symbolic links to
        preserve storage space. This is only allowed when not anonymizing the
        data (i.e., ``anonymize`` must be ``None``).

        .. note::
           Symlinks currently only work with FIFF files. In case of split
           files, only a link to the first file will be created, and
           :func:`mne_bids.read_raw_bids` will correctly handle reading the
           data again.

        .. note::
           Symlinks are currently only supported on macOS and Linux. We will
           add support for Windows 10 at a later time.

    empty_room : mne.io.Raw | BIDSPath | None
        The empty-room recording to be associated with this file. This is
        only supported for MEG data.
        If :class:`~mne.io.Raw`, you may pass raw data that was not preloaded
        (otherwise, pass ``allow_preload=True``); i.e., it behaves similar to
        the ``raw`` parameter. The session name will be automatically generated
        from the raw object's ``info['meas_date']``.
        If a :class:`~mne_bids.BIDSPath`, the ``root`` attribute must be the
        same as in ``bids_path``. Pass ``None`` (default) if you do not wish to
        specify an associated empty-room recording.

        .. versionchanged:: 0.11
           Accepts :class:`~mne.io.Raw` data.
    allow_preload : bool
        If ``True``, allow writing of preloaded raw objects (i.e.,
        ``raw.preload`` is ``True``). Because the original file is ignored, you
        must specify what ``format`` to write (not ``auto``).

        .. warning::
            BIDS was originally designed for unprocessed or minimally processed
            data. For this reason, by default, we prevent writing of preloaded
            data that may have been modified. Only use this option when
            absolutely necessary: for example, manually converting from file
            formats not supported by MNE or writing preprocessed derivatives.
            Be aware that these use cases are not fully supported.
    montage : mne.channels.DigMontage | None
        The montage with channel positions if channel position data are
        to be stored in a format other than "head" (the internal MNE
        coordinate frame that the data in ``raw`` is stored in).
    acpc_aligned : bool
        It is difficult to check whether the T1 scan is ACPC aligned which
        means that "mri" coordinate space is "ACPC" BIDS coordinate space.
        So, this flag is required to be True when the digitization data
        is in "mri" for intracranial data to confirm that the T1 is
        ACPC-aligned.
    overwrite : bool
        Whether to overwrite existing files or data in files.
        Defaults to ``False``.

        If ``True``, any existing files with the same BIDS parameters
        will be overwritten with the exception of the ``*_participants.tsv``
        and ``*_scans.tsv`` files. For these files, parts of pre-existing data
        that match the current data will be replaced. For
        ``*_participants.tsv``, specifically, age, sex and hand fields will be
        overwritten, while any manually added fields in ``participants.json``
        and ``participants.tsv`` by a user will be retained.
        If ``False``, no existing data will be overwritten or
        replaced.


    verbose : bool | str | int | None
        Control verbosity of the logging output. If ``None``, use the default
        verbosity level. See the :ref:`logging documentation <tut-logging>` and
        :func:`mne.verbose` for details. Should only be passed as a keyword
        argument.

    Returns
    -------
    bids_path : BIDSPath
        The path of the created data file.

        .. note::
           If you passed empty-room raw data via ``empty_room``, the
           :class:`~mne_bids.BIDSPath` of the empty-room recording can be
           retrieved via ``bids_path.find_empty_room(use_sidecar_only=True)``.

    Notes
    -----
    You should ensure that ``raw.info['subject_info']`` and
    ``raw.info['meas_date']`` are set to proper (not-``None``) values to allow
    for the correct computation of each participant's age when creating
    ``*_participants.tsv``.

    This function will convert existing `mne.Annotations` from
    ``raw.annotations`` to events. Additionally, any events supplied via
    ``events`` will be written too. To avoid writing of annotations,
    remove them from the raw file via ``raw.set_annotations(None)`` before
    invoking ``write_raw_bids``.

    To write events encoded in a ``STIM`` channel, you first need to create the
    events array manually and pass it to this function:

    ..
        events = mne.find_events(raw, min_duration=0.002)
        write_raw_bids(..., events=events)

    See the documentation of :func:`mne.find_events` for more information on
    event extraction from ``STIM`` channels.

    When anonymizing ``.edf`` files, then the file format for EDF limits
    how far back we can set the recording date. Therefore, all anonymized
    EDF datasets will have an internal recording date of ``01-01-1985``,
    and the actual recording date will be stored in the ``scans.tsv``
    file's ``acq_time`` column.

    ``write_raw_bids`` will generate a ``dataset_description.json`` file
    if it does not already exist. Minimal metadata will be written there.
    If one sets ``overwrite`` to ``True`` here, it will not overwrite an
    existing ``dataset_description.json`` file.
    If you need to add more data there, or overwrite it, then you should
    call :func:`mne_bids.make_dataset_description` directly.

    When writing EDF or BDF files, all file extensions are forced to be
    lower-case, in compliance with the BIDS specification.

    See Also
    --------
    mne.io.Raw.anonymize
    mne.find_events
    mne.Annotations
    mne.events_from_annotations


/home/runner/mne_data/fNIRS-motor-group-bids

To ensure the output path doesn’t contain any leftover files from previous tests and example runs, we simply delete it.

Warning

Do not delete directories that may contain important data!

The data contains annotations; which will be converted to events automatically by MNE-BIDS when writing the BIDS data:

print(raw.annotations)
<Annotations | 92 segments: 15.0 (2), Control (30), Tapping/Left (30), ...>

Finally, let’s write the BIDS data!

bids_path = BIDSPath(subject=subject_id, task=task, root=bids_root)
write_raw_bids(raw, bids_path, overwrite=True)
Loading /home/runner/mne_data/fNIRS-motor-group/sub-01/nirs/sub-01_task-tapping_nirs.snirf
Writing '/home/runner/mne_data/fNIRS-motor-group-bids/README'...
Writing '/home/runner/mne_data/fNIRS-motor-group-bids/participants.tsv'...
Writing '/home/runner/mne_data/fNIRS-motor-group-bids/participants.json'...
Writing '/home/runner/mne_data/fNIRS-motor-group-bids/sub-01/nirs/sub-01_optodes.tsv'...
Writing '/home/runner/mne_data/fNIRS-motor-group-bids/sub-01/nirs/sub-01_coordsystem.json'...
The provided raw data contains annotations, but you did not pass an "event_id" mapping from annotation descriptions to event codes. We will generate arbitrary event codes. To specify custom event codes, please pass "event_id".
Used Annotations descriptions: [np.str_('15.0'), np.str_('Control'), np.str_('Tapping/Left'), np.str_('Tapping/Right')]
Writing '/home/runner/mne_data/fNIRS-motor-group-bids/sub-01/nirs/sub-01_task-Tapping_events.tsv'...
Writing '/home/runner/mne_data/fNIRS-motor-group-bids/sub-01/nirs/sub-01_task-Tapping_events.json'...
Writing '/home/runner/mne_data/fNIRS-motor-group-bids/dataset_description.json'...
Writing '/home/runner/mne_data/fNIRS-motor-group-bids/sub-01/nirs/sub-01_task-Tapping_nirs.json'...
Writing '/home/runner/mne_data/fNIRS-motor-group-bids/sub-01/nirs/sub-01_task-Tapping_channels.tsv'...
Copying data files to sub-01_task-Tapping_nirs.snirf
Writing '/home/runner/mne_data/fNIRS-motor-group-bids/sub-01/sub-01_scans.tsv'...
Wrote /home/runner/mne_data/fNIRS-motor-group-bids/sub-01/sub-01_scans.tsv entry with nirs/sub-01_task-Tapping_nirs.snirf.

BIDSPath(
root: /home/runner/mne_data/fNIRS-motor-group-bids
datatype: nirs
basename: sub-01_task-Tapping_nirs.snirf)

What does our fresh BIDS directory look like?

|fNIRS-motor-group-bids/
|--- README
|--- dataset_description.json
|--- participants.json
|--- participants.tsv
|--- sub-01/
|------ sub-01_scans.tsv
|------ nirs/
|--------- sub-01_coordsystem.json
|--------- sub-01_optodes.tsv
|--------- sub-01_task-Tapping_channels.tsv
|--------- sub-01_task-Tapping_events.json
|--------- sub-01_task-Tapping_events.tsv
|--------- sub-01_task-Tapping_nirs.json
|--------- sub-01_task-Tapping_nirs.snirf

Finally let’s get an overview of the events on the whole dataset

Tapping
trial_type 15.0 Control Tapping/Left Tapping/Right
subject
01 2 30 30 30


We can see that MNE-BIDS wrote several important files related to subject 1 for us:

  • optodes.tsv containing the optode coordinates and coordsystem.json, which contains the metadata about the optode coordinates.

  • The actual SNIRF data file (with a proper BIDS name) and an accompanying *_nirs.json file that contains metadata about the NIRS recording.

  • The *scans.json file lists all data recordings with their acquisition date. This file becomes more handy once there are multiple sessions and recordings to keep track of.

  • And finally, channels.tsv and events.tsv which contain even further metadata.

Next to the subject specific files, MNE-BIDS also created several experiment specific files. However, we will not go into detail for them in this example.

Cite mne-bids#

After a lot of work was done by MNE-BIDS, it’s fair to cite the software when preparing a manuscript and/or a dataset publication.

We can see that the appropriate citations are already written in the README file.

If you are preparing a manuscript, please make sure to also cite MNE-BIDS there.

readme = op.join(bids_root, "README")
with open(readme, encoding="utf-8-sig") as fid:
    text = fid.read()
print(text)
References
----------
Appelhoff, S., Sanderson, M., Brooks, T., Vliet, M., Quentin, R., Holdgraf, C., Chaumon, M., Mikulan, E., Tavabi, K., Höchenberger, R., Welke, D., Brunner, C., Rockhill, A., Larson, E., Gramfort, A. and Jas, M. (2019). MNE-BIDS: Organizing electrophysiological data into the BIDS format and facilitating their analysis. Journal of Open Source Software 4: (1896).https://doi.org/10.21105/joss.01896

Luke, R., Oostenveld, R., Cockx, H., Niso, G., Shader, M., Orihuela-Espina, F., Innes-Brown, H., Tucker, S., Boas, D., Gau, R., Salo, T., Appelhoff, S., Markiewicz, C McAlpine, D., BIDS maintainers, Pollonini, L. (2023). fNIRS-BIDS, the Brain Imaging Data Structure Extended to Functional Near-Infrared Spectroscopy. PsyArXiv. https://doi.org/10.31219/osf.io/7nmcp

Now it’s time to manually check the BIDS directory and the meta files to add all the information that MNE-BIDS could not infer. For instance, you must describe Authors.

Remember that there is a convenient javascript tool to validate all your BIDS directories called the “BIDS-validator”, available as a web version and a command line tool:

Web version: https://bids-standard.github.io/bids-validator/

Command line tool: https://www.npmjs.com/package/bids-validator

Total running time of the script: (0 minutes 3.762 seconds)

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